This was written by a fellow hobbyist on a different forum. I started using it about 2 years ago.
Probiotics have been applied as dietary additives or water additives to improve growth performance and immune response, to improve water quality (by increased waste reduction), and to outcompete pathogenic bacteria.
Bacillus species such as B. subtilis and B. licheniformis are commonly used as probiotics in aquaculture, but there are numerous other bacteria species that are also being utilized within the aquatic industry. In order for probiotics to have any type of positive effect on the fish, they must survive in very large quantity.
Probiotics are usually defined as live microbial feed supplements which beneficially affect the host animal by improving its intestinal microbial balance (Fuller, 1989). Based on this definition, probiotics may include microbial adjuncts that prevent pathogens from proliferating in the intestinal tract (Gatesoupe, 1994). Most probiotics proposed as biological control agents in aquaculture belong to the lactic acid bacteria(LAB). But LAB has some limitations due to having a small antibacterial spectrum. These activities normally inhibit only closely related species of gram-positive microorganisms (Suma et al., 1998). However, almost all the pathogens involved in aquaculture are gram-negative bacteria. Bacillus subtilis, a gram-positive, aerobic, endospore-forming bacterium, would fall under this category, yet this is exactly what two of the fish food manufacturers using probiotics have listed in their ingredients as their only source of probiotic bacteria. The reason for that seems to be that spores of Bacillus strains are thermo-stabile which makes them much easier to use in heat processed pellets.
This is certainly not a new concept in aquaculture, these same type of heterotrophic bacteria have been manufactured for use in septic systems for decades, and there are a number of aquatic related companies that have been marketing these same types of enzymes & micro organisms for just as long.
A couple of fish food manufacturers have stated that their probiotic is based on Bacillus subtilis spores, a good commercial example of that being Calsporin, manufactured by the Calpis Co. Ltd out of Tokyo Japan.
Does it work? Well, according to the more positive studies, yes & no.
http://munin.uit.no/bitstream/handle...pdf?sequence=1
While it does improve the overall feed conversion ratio (FCR) the food used in that study mostly consisted of soybeans & wheat, and while it did have a positive affect on the intestinal microbiota at the initial stages of the feed trial, that apparently soon diminished over time. The longer it was fed, the lesser the positive affect. Also, while it was shown to elevate the expression of some immune related genes, it did not improve the disease resistance of koi challenged with Aeromonas hydrophila, which is one of the most problematic species of Aeromonas found in commercial aquaculture.
So what does that tell the average hobbyist that keeps ornamental species of fish?
Not a whole lot.
If you feed your fish a diet that is high in terrestrial based carbs such as gluten meal, corn, soybeans, wheat, potatoes, rice, etc - adding a probiotic such as Bacillus subtilis in large enough quantity could possibly increase the feed conversion ratio (depending on the species of fish), thereby causing an increase in growth, as well as a reduction in overall waste compared to feeding the same quality of feed, sans the probiotic. It could also help elevate the immune system of the fish, but so could numerous bioactive compounds found in natural raw ingredients such as, Antarctic Krill, Garlic, Spirulina, and micro algae such as Haematococcus pluvialis. Not to mention utilizing key vitamins & trace minerals at levels that far exceed the industry standards. As an example, instead of having a post extrusion level of 140 mg/kg of Vitamin C, having a level of 500+ mg/kg of Vitamin C. (with most of that coming from the raw ingredients themselves) Certainly some of these bacteria have been shown in some studies and using certain species of fish that they can improve the overall feed conversion ratio ....... but of foods that mostly consist of wheat & soybeans.
Of course that's a non issue for anyone that is NOT feeding large amounts of terrestrial based starch derived from corn, wheat, soybeans, potatos, etc to their fish.
Beyond just increasing the feed conversion ratio, and promoting growth, probiotics are also very effective at reducing waste. From an organic reduction standpoint, this isn't exactly cutting edge technology. These exact same heterotrophic bacteria have been used to digest & remove waste from septic systems for decades, the only difference being that in the past no one referred to them as probiotics. And as many of these septic system companies saw the potential for added revenue, they started marketing and selling these non-pathogenic *probiotic* bacteria for ponds, and aquariums. The truth is some are just as safe, and work just as well, as many of the aquatic based products that sell for 10-20 times the price. Some are the exact same bacteria being used, with a different label.
http://www.bio-cat.com/products
I first used a commercial septic tank bacteria approx 10 yrs ago (to kick start some new set ups) and was impressed with the results. After some recent changes in my personal life I found myself looking for ways to reduce water changes, and filter cleaning, beyond just keeping less fish in each tank, or simply adding pothos to reduce nitrates. I wanted to reduce as much time as possible performing regular maintenance, and I believe that I've found the solution.
While this is all very non scientific, here are my results thus far:
1. Water clarity improved immediately. My water has always been clear, but this bacteria took it to a whole new level. It became crystal clear.
2. It has removed all odours from the water. Not that my water stunk prior to this, but one of my tanks that has 3 plecos, and a lot of driftwood, would start to smell within a week of a water change. I could also always smell a hint of garlic in all of my tanks, but not since introducing this bacteria. Overall both the look & smell of my tanks has improved at least somewhat.
3. The overall organics in my filters has been reduced by at least 50%, probably closer to 60% but let's say 50% to be on the safe side. API states 62% reduction in their tests with their Stress Zyme product, and that's probably a very close number to what mine played out to be in my tanks. My 90 gallon is always munged up with debris/waste from my driftwood eating plecos, and after 3-4 weeks the sludge in those filters is as thick as mud. (and stinky!) After 3+ weeks since my last filter cleaning on this tank the one filter barely required cleaning, the other was easily 50% less dirty than normal - and what was left did not smell nearly as bad as it usually does after 3-4 weeks. Filters that previously could only go 3-4 weeks max, can now easily go 6 weeks or more between cleanings.
4. A tank that I have been fighting a cyanobacteria outbreak for a number of months now no longer has any signs of cyanobacteria slime/algae. A different species of algae (in a very small quantity) has managed to now displace it.
With the septic tank bacteria that I use (SeptoBac) I mix 4 teaspoons (1/2 oz) of the dry powder into a liter or so of tank water, stir occasionally for a couple of hours, then pour the solution divided between 3 tanks: two 125 gallons, and one 90 gallon. Most of the solids are left at the bottom of the main container, and not added to my tanks. What does make it in clears off over night. Some of my fish (especially my clown loaches) will eat some of the solids with no ill effect. For Canadian members this product can be found at your local Walmart.
http://septobac.com/
So far total cost is $4.50 that covers a total of 340 gallons x 16 treatments, which works out to a little over $1 a month.
So far this has been a complete success, and that's not even factoring in the competitive exclusion angle with the non pathogenic probiotic bacteria, out-competing any potential pathogenic bacteria. Increasing feed conversion never factored into this experiment as I don't feed foods that are high in low quality raw ingredients, such as excessive amounts of terrestrial based plant matter. (corn, soybeans, potatos, wheat, etc)
Ultimately the goal is to reduce my water changes from 50% weekly, to 50% by-weekly, while at the same time reducing my filter maintenance to half of what it was prior to the start of this experiment.
Species involved in this experiment; Clown Loaches, Snakeheads, Midas, Severum, German Rams, various Corydora, several BN plecos, and an L14 pleco.
A product I have used:
http://acleanearth.com/earthworm_complete_septic.html
Please Note - If anyone tries this in their tanks, please make certain that the products are 100% organic, biodegradable, and non-toxic, and do NOT contain any types of surfactants, perfumes, etc.